Info needed on SUBTRACTIVE HYBRIDIZATION
rah at alf1.ngate.uni-regensburg.de
Mon Jul 24 04:01:35 EST 1995
I hope there is someone out here who knows about or has gathered some
experience with subtractive hybridization. I would like to know if this is a
good way to look for the upregulation of certain genes after a neural trauma.
a) Is there a risk that mRNAs transcribed in big numbers hide other ones that
are only upregulated in a small number. Can this problem be overcome by
subtracting the newly found major mRNA ?
a) Can anyone provide me with a good and reliable protocol
b) What are the main advantages and drawbacks
d) Good books or references
I would higly appreciate any help or useful comments.
Many thanx in anticipation.
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