bovine fragment1-2, prothrombin,blood coagulation
mzb at nirvana.mpg.uni-jena.de
Thu Jun 1 09:40:17 EST 1995
We are a working group in Pharmacological Hemostaseology, blood
We are trying to establish an ELISA/EIA for bovine ! fragment1-2 of Factor
II (prothrombin) to analyze the activation of the coagulation cascade by
different types of materials used in dialysis etc.
Does anybody know a method to isolate "easily" an amount of 1-2 mg
fragment 1-2 (36kD) from bovine plasma (or someone who has been already
successful, possesses antibodies against it)?
We tried it by activating bovine plasma with Russel Viper venom, calcium
chloride and precipitation with barium chloride. For the moment we can t
precipitate the fragment. It seems that the fragments stick to the formed
Thank you for any help.
zur Foerderung der Wissenschaften e.V.
A.G. Pharmakologische Haemostaseologie
Leiter: Prof. Dr. Goetz Nowak
Drackendorfer Strasse 1
e-mail: mzb at leech.mpg.uni-jena.de
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