Help with FSH Bioassay
youngwa at AGRESEARCH.CRI.NZ
Mon Jun 19 15:28:18 EST 1995
For the last 8 months I've been working on an in vitro bioassay for FSH.
However, I have been struggling with a lack of sensativity when dealing with
serum samples which are typically around the 2 ng/ml region.
The assay involves incubating sertoli cells from the testes of 7-10 day old
rats in the presence of FSH. The presence of FSH then causes a dose-dependent
increase in aromatase activity when adrostendione is converted to estradiol
which can be measured to determine the concentration of FSH.
If anyone out there is familiar with this assay and can help me to increase
the sensativity (by decreasing the variability) then please e-mail me
Wayne Young Youngwa at agresearch.cri.nz
AgResearch New Zealand
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