His Tag Purification in Yeast

HPUTZ at BIOTECHNET.COM HPUTZ at BIOTECHNET.COM
Thu Mar 2 15:15:36 EST 1995



Has anybody ever tried to purify His tag proteins which have been
expressed in yeast using the Ni-Agarose column method? Are there
any special measures that must be incorporated when using yeast
to insure efficient binding of the target protein?  Thanks in
advance.

Henry Putz
Bristol-Myers Squibb



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