His Tag Purification in Yeast

TorSm torsm at aol.com
Fri Mar 10 23:27:28 EST 1995


I've done quite a bit of his-tag purification from both sf-9 and
bacterial. I can't imagine that yeast would present any special problems.
Of course, just remember, no EDTA or DTT. I've found that 1-2mM
mercaptoethanol is fine.



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