gentic questions

Michael Cooley szcooley at dale.ucdavis.edu
Fri Mar 10 18:59:18 EST 1995


Oscar Barriuso (barriuso at ux4.cso.uiuc.edu) wrote:
: Can someone please help me answer these questions.

: When cloning foreign DNA, usually a bacteria host that has a mutation in 
: its restriction enzyme gene but a normal modification enzyme gene (r-m+)
: is used.  WHY ?

Originally it was desirable to have the DNA modified if you ten wanted to 
move the plasmid into a new strain which was wild-type for the 
restriction system. It is not as important now because most lab E. coli's 
are restriction minus.

: It is not possible to clone some gene on high copy number plasmids.  Suggest a
: a biological reason why this might be true.



More information about the Bioforum mailing list