szcooley at dale.ucdavis.edu
Fri Mar 10 18:59:18 EST 1995
Oscar Barriuso (barriuso at ux4.cso.uiuc.edu) wrote:
: Can someone please help me answer these questions.
: When cloning foreign DNA, usually a bacteria host that has a mutation in
: its restriction enzyme gene but a normal modification enzyme gene (r-m+)
: is used. WHY ?
Originally it was desirable to have the DNA modified if you ten wanted to
move the plasmid into a new strain which was wild-type for the
restriction system. It is not as important now because most lab E. coli's
are restriction minus.
: It is not possible to clone some gene on high copy number plasmids. Suggest a
: a biological reason why this might be true.
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