Scott R. Burger
burge009 at gold.tc.umn.edu
Wed Nov 8 13:06:53 EST 1995
kkashiha-tky at umin.u-tokyo.ac.jp wrote:
> If anyone has some informations,would you tell me how and why to use DMSO=
>dimethylsulfoxide in moleculer and cellular biology? Any inputs,please!
>Kenichiro Kashihara,a medical student of Tokyo Univ.
One very common use of DMSO is in the cryopreservation of cells. DMSO prevents the formation
of intracellular ice crystals during freezing, making possible long-term frozen storage of
living cells. The following references may be helpful:
Rowley, S. D. Techniques of bone marrow and stem cell cryopreservation and storage. In:
Sacher, R.A., and AuBuchon, JP, eds. Marrow Transplantation: Practical and Technical Aspects of
Stem Cell Reconstitution. Bethesda, MD; American Association of Blood Banks, 1992.
Lovelock, JE, and Bishop, MWH. 1959. Prevention of freezing damage to living cells by
dimethylsulfoxide. Nature 183:1394-5.
Hope this helps.
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