DMSO!?

[Michelle Ford]

In article <DHpEq5.JB4 at umin.u-tokyo.ac.jp>,
kkashiha-tky at umin.u-tokyo.ac.jp wrote:

> Dear Netters
> 
>  If anyone has some informations,would you tell me how and why to use DMSO=
> dimethylsulfoxide in moleculer and cellular biology? Any inputs,please!
> 
> Yours Sincerely
> Kenichiro Kashihara,a medical student of Tokyo Univ.

We use DMSO regularly in our lab for preparing competant bacterial cells
for transformation (with plasmid DNA) - the reference is "Chung et al"
(1989) PNAS vol 86, pp2172-2175.