Help with R&D Systems T cell enrichment column

Damian White d.white at genesis.co.nz
Wed Nov 15 20:56:15 EST 1995


Howdy, all.

I was wondering if anyone out there could lend me some information on R&D
Systems T cell enrichment columns.  The manufacturers claim to be able to
purify T cells to greater than 95% purity, but the best I've obtained so
far is 83%

I've been using murine spleenocytes, ficolled etc. as per the protocol with
the columns.  I think maybe the eluting times/volumes etc. could do with a
little more quantifying.

Thanks in advance,

Damian.

d.white at genesis.co.nz



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