96 minipreps at a time?

Matt Ashby matt at AURORA.XO.COM
Thu Aug 22 23:17:28 EST 1996


Hello,

We would like to know if anyone has a protocol for doing 96 minipreps at 
a time.  We need to be able to start with at leat 1.5ml of ON 
transformed E.coli culture and would like to get around 50ug of plasmid 
DNA from the prep.  The plasmids recovered will be sequenced and used to 
transform S.cerevisiae.  We are aware that 96 well plate are available 
which hold up to 2ml and would like to know if anyone has attempted 
minipreps at this scale before.

Thank you 

Doug Gilliland



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