Help: Transcription & Translation
easyware at gold.interlog.com
Sat Dec 14 23:51:55 EST 1996
Subject: Help: Transcription & Translation
I've been studying basic molecular cell biology for the past semester but
I have a few questions regarding trp operon transcription and translation.
1. When the trp operon of E. Coli is being transcribed under low
tryptophan conditions, is it possible for the ribosome to stall for too
long (if we use a mutant with additional trp encoding codons) at
codons requiring TRP-aminoacetyl tRNA while RNA pol is transcribing?
2. Can a ribosome "fall off" the mRNA by stalling for too long?
3. What affect does increasing the number of "stalling" codons have on
the rate of trancription & translation?
By stalling, I mean that the ribosome must pause at certain codons since
there is a deficiency in an aminoacetyl-tRNA which is required by the
translation of mRNA.
Any help would be greatly appreciated
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