Requesting info on digestion
Aaron David Rosenzweig
recurve at exp2.wam.umd.edu
Sat Feb 3 19:55:01 EST 1996
I'm sending this on behalf of Jeff Rosenzweig who does not
have a net connection:
I am a 10th grade, High School student who is working on a
science fair project. The first part of my project is
concerned with the digestion of substances in the mouth,
stomach and small intestine. While I know which substances
are digested in the three locations
(mouth, stomach, and small intestine), I would like to learn
what chemical reactions occurred.
I am trying to find the formula, chemical equations, and
Lewis Structures for the following:
1. Amylase (mouth) reaction with potato (carbohydrate)
2. Pepsin + HCL (stomach) reaction with boiled egg
whites (protein)
3. Pancreatin (small intestine) reaction with
A. boiled egg whites (protein)
B. butter (fat)
C. potato (carbohydrate)
Procedure: for first experiment
- Added a small sample of the appropriate food to each group
of test tubes.
- Covered each food sample with the appropriate digestive
solution (amylase, pancreatin, or pepsin). Added enough so
that 1/4 to 1/2 of the test tubes are filled. Inserted
stopper and placed in a water bath at 37 degrees C.
- After 24 hours, examined each tube. Cloudiness or a
dissolved food is and indication of enzymatic action.
The second part of my project was to study how bile salts
decreased the reaction time when milk reacted with
pancreatin.
I am trying to find the formula, chemical equations, and
Lewis Structures for the following:
1. milk + bile salt + pacreatin
2. milk + pacreatin
Also, I would like to know why bile salts decreased the
reaction time.
Procedure: for second experiment
- Set up 3 test tubes and added 5 mls of whole milk to each.
- Added 8-10 drops of phenol red solution and shake tubes
until well mixed. If the resulting liquid was not pink I
added NaOH (.l M) drop by drop until pink color remains after
shaking.
- To tube #1, add 5 ml of water and a small quantity of bile
salts (about the amount that would fit on the broad end of a
flat toothpick).
- To tube #2, add 5 ml of pancreatin solution and a small
amount of bile salts.
- To tube #3, add only 5 ml of pancreatin solution.
- Shaked each tube and placed in a hot water bath (37 C).
Recorded the time. Watched for color changes (pink to
yellow) and recorded time of change.
Thank you for any assistance that you can give me.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
If you'd like to send me private e-mail that is ok but
consider also posting or at least doing a cc: to the
newsgroup so that others can benefit :-)
recurve
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