Getting trichocysts to fire in ciliates

Kathleen Anderson vstr18a at
Wed Feb 21 22:55:02 EST 1996

James Mahaffy (mahaffy at wrote:


:      Anyone have a pretty sure fire way to get trichocysts to
: fire on demand from ciliates?  I have a lab, I teach in Zoology 
: where we use Paramecium and some other ciliates (wild varieties I 
: have gathered).  The methods suggested by the lab manual just do not
: work that reliably. They suggest both fountain pen ink and acetic
: acid.  


I've seen it done with the filtrate from crushed geranium leaves. I 
didn't see or do the filtrate prep, but it was yellowish green, and had 
not been heated. It worked quite well and quite quickly.

Hope this helps...

Kathleen Anderson
vstr18a at               

More information about the Bioforum mailing list