Why my DNA after CsCl-ultracentrifugation vanished after EtOH pptn ?

Mark R. Miglarese miglaresemr at pfizer.com
Tue Feb 27 11:17:26 EST 1996

Did you add at least 2.5 volumes of 95-100% ethanol and vortex?  Usually 
there is enough salt (CsCl) for pptation, but you can also add 0.1 
volumes of 3M NaOAc prior to the addition of ethanol.  Also, did you 
incubate the tubes at -20oC for at least 10 min?

-Mark Miglarese

More information about the Bioforum mailing list