Storing in situ hybridization slides

Robert Roeven roeve001 at gold.tc.umn.edu
Fri Jan 5 15:13:20 EST 1996


Hi,

Currently I am involved in in situ hybridizations in alfalfa nodules. I 
use Digoxygenin labeled RNA probes for detection. After the procedure is 
done I dehydrate the sections and mount them with Permount. However when 
I view them under BrightField after that the purple color is gone.

Does anybody have any suggestions on how to permanently store these 
slides without losing the color? Please mail them to me. Thank you very 
much in advance!

Robert Roeven
roeve001 at gold.tc.umn.edu	fax#: +1-612-625-1268

University of Minnesota
411 Borlaug Hall
1991 Upper Buford Circle
St Paul MN 55108
USA


 




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