(no subject)

[xiaoke zhang]

Hello, everyone:

I am a post-doctoral fellow in Kansas University Medical Center. I am now 
working with thioglycollate-elicited mouse peritoneal macrophages. I 
found that, when I used TRIzol Reagent (GIBCO.BRL) to extract RNA from 
these cells, I could not have the RNA pellet dissolved in water, probably 
because of high levels of carbohydrates associated with the RNA. Does 
anyone have other ideas of extracting RNA from this type of cells?

Please e-mail your response to: xzhang at kumc.edu

Thanks.

Charlotte