Quantification of Westerns

[Thomas Weber]

gerard wall wrote:
> 
> I'm currently developing my Westerns using a peroxidase conjugated
> secondary antibody and a chemiluminescence kit but have problems
> quantifying the bands. Has anyone got any suggestions, please?
> 
> wall at biocfebs.unizh.ch

As tough as it is, but if you really want quantitative data you 
should use iodinated Protein A and preferably a Phosphorimager

Thomas