Quantification of Westerns

Thomas Weber tomweber at bway.net
Tue Jan 16 08:46:32 EST 1996

gerard wall wrote:
> I'm currently developing my Westerns using a peroxidase conjugated
> secondary antibody and a chemiluminescence kit but have problems
> quantifying the bands. Has anyone got any suggestions, please?
> wall at biocfebs.unizh.ch

As tough as it is, but if you really want quantitative data you 
should use iodinated Protein A and preferably a Phosphorimager


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