Neutral RED assays

Michael Kolotila x3887 mkolotila at
Fri Jun 7 09:49:37 EST 1996

I used neutral red for several in yeast cidal assay.  Occassionally, I had
a preciptation problem.  As memory serves I usually made up fresh either
daily or on a weekly basis.  Also there is a neutral dye and a neutral red
stain.  I used the dye for my experiments.  I found it easier to make new
stain than to try and determine what was going wrong.  Hope this helps.
Good luck.

 Michael P. Kolotila, Ph.D.        * e-mail: mkolotila at   
 Biotechnology Program Coordinator *          
 Department of Natural Science     * voice :  508-374-3887
 Northern Essex Community College  *
 100 Elliott Way                   * fax   :  508-374-3723
 Haverhill, MA  01830-2399         *        

On Fri, 7 Jun 1996, Dr.M.M.Rajan wrote:

> I having been using neutral red uptake assays for evaluating viable cell
> numbers.
> The solution of neutral red in the medium precipitates. I have been
> incubating the dye solution overnight at 37 degrees. It precipitates even
> then.
> Does anybody face a similar problem. If yes, how does one go about it???
> Thank you
> Yours
> Dr.Rathna Rajan.

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