LEUKEAMIC CYTOGENETICS

HERBERT LUDEWICK 075BERT at CHIRON.WITS.AC.ZA
Fri Jun 7 13:37:59 EST 1996

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COULD ANYONE PLEASE HELP US? 

WE WERE USING TC ARRESTING SOLUTION TO ARREST LEUKEAMIC CELL CULTURES, 
AND FOUND THAT THIS WORKED VERY WELL FOR OVERNIGHT EXPOSURE AS WELL AS 
TWO HOURS AND 30 MINUTE EXPOSURE. DUE TO THE DISCONTINUING OF THIS 
PRODUCT WE HAVE TO SWITCH TO COLCEMID OR COLCHICINE. COULD YOU PLEASE 
SUGGEST THE BEST ALTERNATIVES AS WELL AS THE CONCENTRATIONS AND EXPOSURE 
TIMES (INCLUDING OVERNIGHT EXPOSURE).

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