ARGH! ****GC RICH AREA********
Calvino Ka-wing Cheng
ckwcheng at acs4.acs.ucalgary.ca
Mon Jun 10 22:52:39 EST 1996
I was wondering if you could help me. I'm doing PCR on a
plasmid in the attempt to "site specifically" mutagenise the
resulting enzyme. The residue which we picked is exactly in the
middle of a GC rich region which encodes for it.
1. Using PCR, is there a way of unravelling the GC rich primer
(56% GC)? Can we heat it up?
2. We are using blunt end ligation. Is there another way of
creating a primer in a different spot and then using sticky end
ligation? If so, could you advise us.
Any suggestions? If you want clarification, e-mail me. Thanks
for your anticipated help.
More information about the Bioforum