LPS inhibition of CYCLOHEXAMIDE SUPERINDUCTION

[ian]

I am currently studying a gene which is LPS inducible and is also 
cyclohexamide (CHX) superinducible, no problem. However, when LPS and 
CHX are added simultaneously, gene expression is completely switched 
off. I would expect it to at least return to the level superinduced by 
CHX, however in the presence of CHX, LPS seems to act as an inhibitor 
rather than an inducer.  Has anyone seen this before, or are there any 
suggestions as to why it is occuring?

Robert Passey