A question about in situ hybridization

Martin Downes martind at magi.com
Wed Mar 13 09:45:15 EST 1996


I need some help.

I am trying to do in situ hybridization on parrafin embedded
sections with a viral RNA target. I have used cRNA probes 300
900 + 1400 bas and oligo probes. These probes work fine in
other systems but connot get a positive signal in the above
system.

Has anyone else experienced this problem and what did you do?
Any suggestions would be welcome.

Thanks,

Mary Downes
Ottawa, Canada



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