Help with SDS Gel Mixture

Yuhsaku Godai [Ronin] bc15 at midway.uchicago.edu
Thu Mar 14 15:16:02 EST 1996


I'm trying to run my first SDS-PAGE gel but can't seem to get the gel 
solution correct.  From the Molecular Cloning lab manual, I've been 
trying to make 5 ml of a 10% gel but it's not polymerizing completely.  
Although addition of either more ammonium persulfate or TEMED seems to 
make it gel properly, why isn't the amounts given in the manual correct?  
Are there other factors I need to take into consideration?  Any help 
appreciated.  Thanks.

-Ben
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