Lymphocytes cultivation

chan siu chiu bcscc at
Wed May 1 11:32:27 EST 1996

I isolate lymphocytes from human blood using gradient centrifugation 
method.I put the isolated lymphoctes in the RPMI1640 plus 10% FBS in 
the incubator. I found about 20-30% of the lymphocytes their shape 
changed to irregular from initial spherical shape. Would you suugest
any methods and medium  for good maintainence of the lymphocytes in 
the incubator ?

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