Genomic labelling in-situ of plant DNA
abrdlher at reading.ac.uk
Wed May 29 13:26:07 EST 1996
I am working with wheat.
I want to intercross two lines, but I want to keep track, in the very
early embryo and endosperm development, of the parental chromosomes.
I thought of introducing tritiated thimidine into the spikes, previous to
Any other way of labelling, not involving tritium?
Any thoughts as to what would be the best way to introduce the labelled
compound into the plant? I thought of putting a fine thread through the
stem, wetted in the labelling solution, and let the transpiration flow
carry the label to the pollen mother cells, and to the eggs. Any better ways?
Thank you for any help
Nacho de las Heras
University of Reading
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