why don't we freeze 35-S Autorads ?

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Sun Nov 24 12:33:20 EST 1996

mac-biocomputing1user (user at mac-biocomputing1.embl-heidelberg.de) wrote:
> Hi ...this week I followed the "cool" discussion about why do we freeze
> autorads and while I was reading all the answers I was wondering why
> don't we freeze autorads when we expose dried polyacrilammide gel for
> sequencing where the isotope used is usually 35-S instead of 32-P !!!???


> 2) It is a metter of the particular isotope (35-S or 32-P) and so of the
> different decay and particles emitted

Yup. 35-S emits low-energy electrons which don't need intensifying screens.
If you don't use screens, you don't have to freeze your gel.


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP3 */
/* "Science is the game we play with God to find out what His rules are."  */

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