procaryotic transcription terminator: help wanted
Jean-Yves Paquet
paquet at biq.fundp.ac.be
Thu Oct 17 09:56:36 EST 1996
Hi,
We are currently working on promotors regulated by a response regulator in
a bacteria. We have to make some constructions with a pKK-type vector a
it's important for us to have a transcription terminator after our gene.
There is one terminator but it seems to be in the wrong polarity. So, my
question is:
Are the transcription terminator oriented, I mean, if there is one
terminator, does it act from 5' -> 3' AND also from 3' -> 5' ?
Thanks in advance...
--
Jean-Yves PAQUET
Unité de recherche en Biologie Moléculaire
Facultés Universitaires de Namur, Belgium
jean-yves.paquet at fundp.ac.be
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