advice needed for cyrosectioning work.

Khoo Keng Meng medp6023 at leonis.nus.sg
Fri Sep 13 23:07:13 EST 1996


I am currently trying to get good cyrosections from the whole eye (rat).
Does anybody know a tried and tested way to do cyrosections of the eye?
The protein i am trying to isolate is membrane bound. Do i need to perform
any fixation? ...or sucrose immersion?
thanks.

k.m.




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