Q: Necrosis vs. Apoptosis

[Jackie E. Kylander]

In article <33443CFE.64D8 at julian.uwo.ca>, David O'Neill
<doneill2 at julian.uwo.ca> wrote:

>Hi again,
>I'm trying to differentiate between necrotic endothelial cells and
>apoptotic endothelial cells.  Other than morphological analysis of
>blebbing etc. are there any immunohistochemical markers capable of this
>distinction.  For instance, a marker for two differing types of DNA
>fragmentation?  Due to the loss of membrane integrity during necrosis, I
>wonder if it is physically possible to identify these cells at all.  
>Diffuse counterstaining (of necrotic cells) against an apoptotic marker
>won't help me as I need to identify (and count) capillaries undergoing
>necrosis.  
>Any suggestions are appreciated.  Have a great day!
>Thanks,
>Dave.

Dave, I have no suggestions for you only the best of luck. I've tried
several of the commercial "apoptosis" detection kits but ALL have
overestimated the number of apoptotic cells in our model (tracheal injury
and repair). Each kit has given way over 50% detection--a number that
cannot be backed up by any other method (PC10 staining, morphology, BRdU,
cell nucleus counts, etc, etc.) we suspect a problem with
fixation/embedment but cannot seem to pin it down.

-- 
Jackie E. Kylander <jek at med.unc.edu>
A/OSB