Quantifying Peptide attachment to a substrate

[Dennis Templeton]

In article <5hh011$6ug at swsu65.swmed.edu>, James Clift
<clift at utsw.swmed.edu> wrote:

> Hey. I have got a problem here:
>         I am trying to grow hepatocytes on a polymer membrane. 
>         I attach the adhesion peptides: YIGSR, RGD to it using certain 
>  standard protocols. 
> 
>         How do I find out if the peptides are attached, and how
>  do I quantify the attachment. The peptides are being used in
>  nanograms/ml concentration. And this is a solid substrate.
>  
>  Forward suggestions to 
>       mariappan at utsw.swmed.edu
>   or  carlisle at utsw.swmed.edu
> 
> Thanking you,
> Yours
> M.M.Rajan

We have had amino acid analysis done on bead-bound peptides. It is
relatively cheap ($25) and very quantitative.

Dennis

Dennis Templeton
Institute of Pathology
CWRU School of Medicine
djt2 at po.cwru.edu
http://129.22.87.13