The human genom - how is it distributed?

Mr RJ Dickinson rdickins at
Thu Apr 24 05:35:17 EST 1997

Bill Tivol / Susan Hogarth debate

regarding the discussion of whether or not ORFs can be distributed on both
strands of the mammalian genome such that a given sense strand for one
gene could be the antisense of another - what about genes for which there
is demonstrable antisense transcription of a coding sequence, such as
(from memory) the bFGF (at least I think it is bFGF) gene with the
antisense transcript gfg (attempted cut and paste of relevant MEDLINE

UI  - 95091784
AU  - Knee RS
AU  - Pitcher SE
AU  - Murphy PR
TI  - Basic fibroblast growth factor sense (FGF) and antisense (gfg) RNA
      transcripts are expressed in unfertilized human oocytes and in
      differentiated adult tissues.
GS  - gfg
GS  - bFGF
SI  - GENBANK/L31408
DP  - 1994 Nov 30
TA  - Biochem. Biophys. Res. Commun.
PG  - 577-583
IP  - 1
VI  - 205
AB  - Basic fibroblast growth factor (bFGF) is a highly conserved and
      ubiquitously distributed mitogen. In amphibian oocytes bFGF mRNA is
      regulated post-transcriptionally by interaction with an antisense
      transcript. We used reverse transcription-polymerase chain reaction
      PCR) and Northern hybridization to determine the presence of bFGF
      its antisense RNA (gfg) in unfertilized human oocytes and postnatal
      differentiated tissues. BFGF and gfg transcripts were co-expressed
      many tissues, with bFGF transcripts (7, 3.7 and 1.8 kb) being more
      abundant than the gfg transcript (1.5 kb) in 8 of 16 tissues
      Sense and antisense expression was approximately equal in kidney and
      colon, while in heart, liver, skeletal muscle and testis gfg
      transcripts predominated. RT-PCR demonstrated the presence of bFGF
      gfg transcripts in unfertilized oocytes where the antisense
      was present in excess of the sense transcript. These findings
suggest a
      role for gfg in regulation of bFGF expression.
AD  - Department of Physiology and Biophysics, Dalhousie University,
      Nova Scotia, Canada.
SO  - Biochem. Biophys. Res. Commun. 1994 Nov 30;205(1):577-583

surely these events, although admittedly rare, ought to be mentioned
(isn't there something similar with c-myc regulation as well)?

Rob Dickinson
DHA, University of Oxford, England

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