The human genom - how is it distributed?
Mr RJ Dickinson
rdickins at hgmp.mrc.ac.uk
Thu Apr 24 05:35:17 EST 1997
Bill Tivol / Susan Hogarth debate
regarding the discussion of whether or not ORFs can be distributed on both
strands of the mammalian genome such that a given sense strand for one
gene could be the antisense of another - what about genes for which there
is demonstrable antisense transcription of a coding sequence, such as
(from memory) the bFGF (at least I think it is bFGF) gene with the
antisense transcript gfg (attempted cut and paste of relevant MEDLINE
UI - 95091784
AU - Knee RS
AU - Pitcher SE
AU - Murphy PR
TI - Basic fibroblast growth factor sense (FGF) and antisense (gfg) RNA
transcripts are expressed in unfertilized human oocytes and in
differentiated adult tissues.
GS - gfg
GS - bFGF
SI - GENBANK/L31408
DP - 1994 Nov 30
TA - Biochem. Biophys. Res. Commun.
PG - 577-583
IP - 1
VI - 205
AB - Basic fibroblast growth factor (bFGF) is a highly conserved and
ubiquitously distributed mitogen. In amphibian oocytes bFGF mRNA is
regulated post-transcriptionally by interaction with an antisense
transcript. We used reverse transcription-polymerase chain reaction
PCR) and Northern hybridization to determine the presence of bFGF
its antisense RNA (gfg) in unfertilized human oocytes and postnatal
differentiated tissues. BFGF and gfg transcripts were co-expressed
many tissues, with bFGF transcripts (7, 3.7 and 1.8 kb) being more
abundant than the gfg transcript (1.5 kb) in 8 of 16 tissues
Sense and antisense expression was approximately equal in kidney and
colon, while in heart, liver, skeletal muscle and testis gfg
transcripts predominated. RT-PCR demonstrated the presence of bFGF
gfg transcripts in unfertilized oocytes where the antisense
was present in excess of the sense transcript. These findings
role for gfg in regulation of bFGF expression.
AD - Department of Physiology and Biophysics, Dalhousie University,
Nova Scotia, Canada.
SO - Biochem. Biophys. Res. Commun. 1994 Nov 30;205(1):577-583
surely these events, although admittedly rare, ought to be mentioned
(isn't there something similar with c-myc regulation as well)?
DHA, University of Oxford, England
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