Pichia pastoris expression systems

William Reisdorf reisdorf at GPC.IBC.WUSTL.EDU
Thu Apr 24 13:48:44 EST 1997


Our lab is considering using the Zeocin-TM Pichia expression system
from Invitrogen and we're looking for any helpful comments from
others who may have used this particular system. Positive and negative
experiences would be appreciated. Our goal is to express IgSF domains
from a receptor tyrosine kinase, and so far our luck in refolding
them from E. coli inclusion bodies has been poor. We hope that the
disulfides will be able to form better and that glycosylation may
also help with the stability of our recombinant protein. 

William Reisdorf (postdoc)
Washington University
Biochem & Molec Biophys and
Inst for Biomed Computing
reisdorf at ibc.wustl.edu



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