PROGRAM OF IMMUNOFLUORESCENCE COURSE

Paolo Castano clsmteam at imiucca.csi.unimi.it
Thu Apr 24 10:43:57 EST 1997


Advanced International Immunofluorescence Course	
		Gargnano '97 (Italy)

The Advanced International Immunofluorescence Course is a
post-doctorate theoretical/practical course, with propedeutical
lectures and practical stages on traditional and confocal
immunofluorescence microscopy and image and ion analysis. 
	The course will take place in Gargnano (Lake of Garda) from 7
to 10 October 1997. Further information and registration details will
be found at the following Web address

http://imiucca.csi.unimi.it/endomi/ACIF.html

Program:

Morning	Tuesday, October 7, 1997

9.00-10.00	Registration of partecipants

10.00-10.45	AN INTRODUCTION TO 	
		IMMUNOCYTOCHEMISTRY AND
		IMMUNOFLUORESCENCE  (I.Barajon)

10.45-11.15	Coffee Break

11.15-12.00	LIGHT SOURCES AND FLUOROCHROMES 
		(G. Bottiroli)

12.00-12.45	THE USE OF FLUORESCENCE MICROSCOPE 	(C.Rumio)

13.00-14.30	Work lunch

Afternoon

14.00-14.45	FLUORESCENCE PHOTOMICROGRAPHY 	(P.Castano)

14.45-16.00	Practical stages:  Photomicrography 

16.00-16.30	Coffee Break

16.30-17.30	Practical stages:  Photomicrography 

Morning  	Wednesday, October 8, 1997

  9.00 - 9.45	IMMUNOCYTOCHEMISTRY AGAINST 	IMMUNOFLUORESCENCE 	
		(C.J.F.Van Noorden)

9.45-10.30	APPLICATION OF DIGITAL IMAGE ANALYSIS TO FLUORESCENCE
MICROSCOPY (A.Remuzzi)

10.30-11.00	Coffee Break

11.00-11.45	PRINCIPLES OF CONFOCAL MICROSCOPY (G.J.Brakenhoff)

11.45-12.30	GFP AND Ca 2+  ANALYSIS  (R. Rizzuto)

12.30-14.00	Work Lunch

Afternoon

14.30-16.00	Practical stages: Photomicrography, Confocal 		
		Microscopy, Ca Analysis, Image Analysis.

16.00-16.30	Coffee Break

16.30-17.30	Practical stages:
		Photomicrography, Confocal Microscopy, Ca Analysis,
Image Analysis.
Evening

21.00-23.00	Illustration and discussion of photomicrographies.

Morning	Thursday, October 9, 1997

 9.00 - 9.45	IMMUNOFLUORESCENCE FOR CELL 	CULTURES (C.Tacchetti)

9.45-10.30	IMMUNOFLUORESCENCE ON THICK 	SAMPLES (S.Modina)

10.30-11.00	Coffee Break

11.00-11.45	MULTIPHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
(A.Dixon)

11.45-12.30	MULTIPLE FLUORESCENCE  (A. Entwistle)

12.30-14.00	Work Lunch

Afternoon 

14.00-17.00	Visit to the Vittoriale of G.D'Annunzio

17.00-19.30	Practical stages: Photomicrography, Confocal 	
		Microscopy, Ca Analysis, Image Analysis.

Evening

20.30	All-together Dinner in a typical Restaurant

Morning	Friday, October 10, 1997

 9.00 - 9.45	CONFOCAL MICROSCOPY AND FLUORESCENCE(A.Entwistle)

 9.45 -11.00	Round Table:  THE MULTI-DIMENSIONAL MICROSCOPY
(G.J.Brakenhoff, A.Entwistle, 	P.Castano, A.Remuzzi, C.J.F.Van
Noorden)

11.00-11.30	Coffee Break

11.30-13.30	Practical stages:  Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.

13.30-14.30	Work Lunch

Afternoon

14.30-17.00	Practical stages:  Photomicrography, Confocal
Microscopy, Ca Analysis, Image Analysis.

17.30	End of works.



Thank you
Paolo Castano



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