Reg: Chelex extraction for DNA sequencing?

Jannik Helweg-Larsen jhelweg at INET.UNI2.DK
Thu Dec 18 16:30:32 EST 1997


Does anybody our there have any experience with the use of Chelex extracted DNA for sequencing?

For routine PCR diagnostics of Pneumocystis carinii we have used  Chelex 100 with succes.

I plan to do rRNA sequencing, will Chelex extracted DNA work for cloning and sequencing? Is it stable in the freezer?

Jannik Helweg
Dept of Infectious Diseases, Hvidovre Hopital, Denmark
jhelweg at inet.uni2.dk





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