Hopkins Grad Seeks Post-Doc in AIDS/HIV Research

Michael A. Egan egan at welchlink.welch.jhu.edu
Mon Jan 6 10:47:24 EST 1997


                                Department of Molecular Biology and Genetics
                                Johns Hopkins University School of Medicine
                                Ross Research Bldg., Rm. 1050
                                720 N. Rutland Ave.
                                Baltimore, MD 21205



January 5th, 1997

To whom it may concern:

I am writing in regards to potential post-doctoral / entry level positions
that may be available in the areas of basic science AIDS research and HIV
vaccine development.

I am a 5th year Ph.D. candidate in the Biochemistry, Cellular and Molecular
Biology Training Program at the Johns Hopkins University School of Medicine.  I
plan to complete my graduate program in the spring/summer of 1997.

My research, under the direction of Robert F. Siliciano M.D. Ph.D., has been
focused in two areas related to HIV/AIDS research.  I developed a novel method
(AIDS Research and Human Retroviruses, 10: 1423, 1994) for the detection
and evaluation of cytolytic T-cell responses to candidate AIDS vaccines
being tested
in Phase I clinical trials at the Center for Immunization Research here at 
Johns Hopkins (J. Infect. Dis. 171: 1623, 1995).  This method is now widely
used in the testing of AIDS vaccines.  

More recently my thesis work has focused on developing a better understanding
of the role of a highly conserved YXXL internalization motif within the
envelope glycoprotein gp41 of HIV-1 in viral assembly, host immune recognition,
and CD4 cell loss (J. Virol., 10:6547-6556 (1996)). 

I seek a post doctoral / entry level position in the area of basic
sciences AIDS research and HIV vaccine development. My nine years research
experience, my excellent writing and verbal skills, along with my proven
ability to publish in peer reviewed journals makes me an excellent
candidate.   

I welcome an opportunity to discuss my capabilities with respect to
opportunities that may exist in this area.  I have enclosed a copy of my
curriculum vitae along with the name and addresses of three references for
your information.  

Please feel free to contact me by mail at the above address or by telephone or
E-mail:

        Phone: 410-955-7757
        Fax: 410-955-0964
        email: egan at welchlink.welch.jhu.edu
   http://www.welch.jhu.edu/~egan

Sincerely,


Michael A. Egan
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                                CURRICULUM VITAE


Name:                           Michael A. Egan

Current Appointment:            Ph.D. Candidate, Graduate Program in    
                                Biochemistry, Cellular and Molecular Biology,   
                                Johns Hopkins University, School of
Medicine,                                           
                                Baltimore, MD

Office Address:                 Johns Hopkins University School of Medicine,
                                Department of Medicine
                                Rm. 1050 Ross Research Bldg.
                                720 N. Rutland Ave.
                                Baltimore, MD 21205
                                Phone: 410-955-7757
                                Fax: 410-955-0964
                                e-mail: egan at welchlink.welch.jhu.edu
                                http://www.welch.jhu.edu/~egan

Home Address:                   3721 Rexmere Rd.
                                Baltimore, MD 21218
                                Phone 410-366-3268

EDUCATION

1992-present                    Ph.D. Candidate, Graduate Program in    
                                Biochemistry, Cellular and Molecular Biology    
                                (BCMB), Johns Hopkins University, School of     
                                Medicine, Baltimore, MD

1987-1992                       B.S., Chemistry, Magna Cum Laude, 
                                Northern Arizona University, Flagstaff, AZ


                            HONORS AND AWARDS

Undergraduate                   Magna Cum Laude in Chemistry
Education                       Grade Point Average 3.80/4.00
                                NSF Summer Research Fellowship
                                Golden Key National Honor Society
                                Phi Lambda Upsilon Chemistry Honor Society
                                Phi Kappa Phi Honor Society
                                Dean's List seven semesters

Post Graduate                   Fellowship, Graduate Program in Biochemistry,   
Education                       Cellular and Molecular Biology (BCMB), Johns 
                                Hopkins University School of Medicine



                                WORK EXPERIENCE

1988-1991                       Undergraduate Chemistry lab teaching assistant,
                                Northern Arizona University

Summer 1991                     NSF Research Fellowship, University of Georgia

1983-1987                       Enlistment in the U.S. Marine Corps, honorable 
                                discharge.


                           RESEARCH EXPERIENCE

1992-Present                    Elucidating the role of the YXXL
internalization 
                                motif within the envelope protein (gp41) of 
                                HIV-1 in viral assembly, immune
recognition, and 
                                CD4 cell loss.  Developed a novel method,
now                                   
                                widely used in the AIDS field, for the
detection        
                                of (HIV-1)-specific CTL responses in human 
                                vaccine recipients. In a Phase I
clinical                                        
                                trial evaluated the ability of an experimental 
                                HIV-1 vaccine to elicit HIV-specific CTL 
                                responses in sero-negative volunteers.

                                Laboratory of Robert F. Siliciano, 
                                Department of Medicine,
                                Johns Hopkins University School of Medicine.

1991-1992                       Using semi-empirical computational methods to 
                                calculate the structure and electronic 
                                properties of the mono-boro-analogs of 
                                acetylcholine. 

                                Laboratory of Dr. Robert W. Zoellner, 
                                Department of  Chemistry, 
                                Northern Arizona University

NSF 1991 Summer                 Mutagenesis of murine ferrochelatase in looking 
Fellowship                      for molecular mimicry in erythropoietic 
                                protoporphyria.

                                Laboratory of Dr. Harry A. Dailey, Department of
                                Microbiology, University of Georgia

1988-1992                       The effect of endurance training on glucose 
                                metabolism.

                                Laboratory of Dr. Scott Savage and Dr.
Hans M.          
                                Gunderson, Exercise Physiology Lab, Northern 
                                Arizona University



SKILLS

Trained in standard molecular biology techniques, PCR, SDS-PAGE, Western
Blot, Pulse Chase Analysis, the use of Fluorescence Activated Cell Sorter
analysis (FACS), all aspects of human tissue culture, and optimization and
implementation of standard immunological assays (Cr51 release, CTL assays,
ADCC, ELISA). Five years experience in the handling and use of high titer
infectious material (HIV-1, vaccinia virus, EBV) under Biohazard Level 3
(P3) laboratory conditions. Computer trained with experience programming
in FORTRAN, C/C++, HTML. Skilled in the use of DOS/Windows, Mac and Unix
operating systems. Able to efficiently access and publish information on
the WWW and Internet. 


PUBLICATIONS

1. Michael A. Egan and Robert W. Zoellner.  Structural and Electronic
Characteristics of the Monoboro-Analogs of the Acetylcholine Cation As
Determined by the Semiempirical MNDO Computational Method.  J. Org. Chem.,
58:1719-1729 (1993).

2. M.N. Lubaki, Michael. A. Egan, Robert. F. Siliciano, Kent. J. Wienhold,
and Robert. C. Bollinger. A Novel Method for the Detection and Expansion
of HIV Type 1-Specific Cytolytic T Lymphocytes. AIDS Res. Hum.
Retroviruses, 10:1423-1427 (1994).

3. Michael A. Egan, Wendy A. Pavlat, James Tartaglia, Enzo Paoletti, K. J.
Wienhold, Mary Lou Clements, and Robert F. Siliciano. Induction of Human
Immunodeficiency Virus Type 1 (HIV-1)-Specific Cytolytic T Lymphocyte
Responses in Seronegative Adults by a Nonreplicating,
Host-Range-Restricted Canarypox Vector (ALVAC) Carrying the HIV-1MN env
Gene, J. Infect. Dis., 171:1623-1627 (1995).

4. Robert. C. Bollinger, Michael. A. Egan, Tae-Wook Chun, Bonnie
Mathieson, and Robert F. Siliciano. Cellular Immune Responses to HIV-1 in
Progressive and Non-Progressive Infections. AIDS, 10:1-12 (1996).

5. Michael A. Egan, Lucy M. Carruth, Jennifer F. Rowell, Xiaofang Yu, and
Robert F. Siliciano. HIV-1 Envelope Protein Endocytosis Mediated by a
Highly Conserved Intrinsic Internalization Signal in the Cytoplasmic
Domain of gp41 Is Suppressed in the Presence of the Pr55gag Precursor
Protein, J. Virol., 70:6547-6556 (1996).

Abstracts

1. Michael A. Egan and Robert W. Zoellner.  The Mono-Boro-Analogs of
Acetylcholine: Structural and Electronic Comparisons to the Parent
Acetylcholine Using the MNDO Computational Method.  Annual American
Chemical Society Meeting, San Francisco, CA, April 1992.

2. Michael A. Egan, Kent Weinhold, Enzo Paoletti, James Tartaglia, Mary
Lou Clements, Wendy Pavlat, and Robert F. Siliciano.  Cytotoxic T
Lymphocyte Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125,
HIV-1 gp160 MN) in HIV-1 Uninfected Adult Volunteers. The 6th Annual
Conference on Advances in AIDS Vaccine Development, Alexandria, VA, 
October 30th - November 4th, 1993.

3. Michael A. Egan, Wendy Pavlat, James Tartaglia, Enzo Paoletti, Robert
F. Siliciano and Mary Lou Clements.  Induction of HIV-1-specific Cytotoxic
T Lymphocyte Responses in Seronegative Adults by a Non-Replicating, Host
Range Restricted Recombinant Canarypox Vector
Carrying the HIV-1 env Gene.  The 7th Annual Conference on Advances in
AIDS Vaccine Development, Reston VA, November 6th-10th, 1994.

4. Michael A. Egan, Wedy Pavlat, James Tartaglia, Enzo Paoletti,  Mary Lou
Clements and Robert F. Siliciano.  Cytotoxic T Lymphocyte Responses to
Recombinant Canarypox-gp160 MN (ALVAC vCP125, HIV-1 gp160 MN) in HIV-1
Uninfected Adult Volunteers.  The Frank B. Polk Symposium on AIDS,
Baltimore, MD, April 15th-16th, 1994.

5. Michael A. Egan, Jennifer F. Rowell, Robert F. Siliciano and Lucy M.
Carruth. The Role of the gp41 (YXXL) Internalization Motif in HIV-1
Assembly.", Third Conference on Retroviruses and Opportunistic Infections,
Washington, DC, January 28th - February 2nd, 1996. 
 
REFERENCES

                        Robert F. Siliciano, M.D., Ph.D.
                        Assistant Professor, Department of Immunology
                        Johns Hopkins University School of Medicine
                        Rm. 1049, Ross Research Bldg.
                        720 N. Rutland Ave.
                        Baltimore, MD  21205
                        Office: 410-955-2958
                        Fax:    410-955-0964
                        e-mail: rsilicia at welchlink.welch.jhu.edu

                        Robert C. Bollinger, M.D., M.P.H.
                        Assistant Professor, Division of Infectious Disease
                        Johns Hopkins University School of Medicine
                        Rm. 1159, Ross Research Bldg.
                        720 N. Rutland Ave.
                        Baltimore, MD  21205
                        Office: 410-614-0936
                        Fax:    410-955-7889
                        e-mail: rcb at welchlink.welch.jhu.edu

                        Xiao-Fang Yu, M.D., Ph.D.
                        Department of Molecular Biology and Immunology
                        Johns Hopkins University
                        School of Hygiene and Public Health
                        615 N. Wolfe, St.
                        Baltimore, MD 21205
                        Phone:  410-955-3768
                        Fax:    410-955-0105
-----------------------------------------------------------------------------

-- 
Michael A. Egan
Department of Molecular Biology and Genetics
Ross Research Bldg., Rm. 1050
Johns Hopkins University
720 N. Rutland Ave.
Baltimore MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.edu
http://www.welch.jhu.edu/~egan



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