Hemoglobin measurement at 540nm or 430nm?
According to some literature the release of hemoglobin from erythrocytes
after the addition of surfactants is measured at 540 nm. We measure a
much higher absorption at 430 nm compared to 540 which is consistent
with the fact that hemoglobin is red. Does anybody know why I should
measure hemoglobin release at 540 nm in stead of 430 nm?
Thanks in advance, Leendert Hamoen