Hemoglobin measurement at 540nm or 430nm?

Leendert Hamoen l.w.hamoen at biol.rug.nl
Fri Jan 31 10:46:42 EST 1997

Hemoglobin measurement at 540nm or 430nm?

According to some literature the release of hemoglobin from erythrocytes 
after the addition of surfactants is measured at 540 nm. We measure a 
much higher absorption at 430 nm compared to 540 which is consistent 
with the fact that hemoglobin is red. Does anybody know why I should 
measure hemoglobin release at 540 nm in stead of 430 nm?

Thanks in advance, Leendert Hamoen

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