Free software for data analysis and simulation

Petr Kuzmic pkuzmic at
Tue Jul 22 10:50:32 EST 1997


Program DYNAFIT [1] for the statistical analysis of enzyme kinetic and
receptor binding data can be downloaded the BioKin Ltd. web site [2].

The program is useful for fitting the following types of experimental data:

	* initial reaction velocities in enzyme assays;
	* equilibrium composition of complex mixtures;
	* reaction progress (concentration or a related quantity vs. time).

The reaction mechanism is described symbolically, in the form of chemical
stoichiometric equations.  For example, tight-binding, mixed-type,
noncompetitive enzyme inhibition is notated as follows (E...enzyme,
S...substrate, I...inhibitor):

		E + S <===> ES
		ES ---> E + P
		E + I <===> EI
		ES + I <===> ESI
		ESI ---> EI + P

A reviewer [3] wrote: "Perhaps the most impressive feature is the extensive
set of sample script files that serves as a tutorial for enzyme kinetics
and receptor-ligand reactions. If you do any kind of kinetic data analysis
as part of your research program, be sure to download DynaFit from the
BioKin Web site."


[1] Kuzmic, P. (1996) Anal. Biochem. 237, 260-273. "Program DYNAFIT for the
Analysis of Enzyme Kinetic Data: Application to  HIV Proteinase" 



Petr Kuzmic, Ph.D.      |          (608) 256-4790
BioKin Consulting       |      fax (708) 256-1269
P.O. Box 8336           |      pkuzmic at
Madison WI 53708        |

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