Paraformaldehyde and autofluorescence.

IMMUNO-SHERBROOKE immunolo at COURRIER.USHERB.CA
Wed Mar 5 15:01:51 EST 1997


	I perform immunophenotyping of human lymphocytes by flow cytometry.
I use 1% paraformaldehyde solution (PFA; Sigma) in PBS for cell fixation.
The autofluorescence of fixed cell exited at 488nm is increased 6-7 fold
comparing to nonfixed control.
	1. Can someone suggest me an alternative PFA source which gives
minimal autofluorescence?
	2. Can someone suggest me a post-fixation protocol which reduces
PFA-fixed cell autofluorescence?
				Thank you.
				Dr. L. Volkov






More information about the Bioforum mailing list