storing antibodies

[Steven Pirie-Shepherd]

John McGraw (john at unixg.ubc.ca) wrote:
: What is the best way to store antibodies for extended periods of time? 
: Should they be stored at -80 C or at -20 C.  I have heard it is better to
: store them in glycerol (1:1) to perserve them for a longer period of time.



1. Purified monoclonal and polyclonal antibodies-store 1:1 in glycerol at
-70C

2. Polyclonal antiosera , -20C is fine (the albumin provides protection).

3. Conjugated Abs (eg HRP or AP..tricky, if you made them , store them
1:1 in glycerol, if you bought them, follow manufacturers instructions.

This always worked for me.

__________________________________________________
Steven Pirie-Shepherd	srps at galactose.mc.duke.edu
919.684.8986		919.684.8689 FAX
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