Quantifying Peptide attachment to a substrate

James Clift clift at utsw.swmed.edu
Fri Mar 28 12:44:01 EST 1997


Hey. I have got a problem here:
	I am trying to grow hepatocytes on a polymer membrane. 
	I attach the adhesion peptides: YIGSR, RGD to it using certain 
 standard protocols. 

	How do I find out if the peptides are attached, and how
 do I quantify the attachment. The peptides are being used in
 nanograms/ml concentration. And this is a solid substrate.
 
 Forward suggestions to 
      mariappan at utsw.swmed.edu
  or  carlisle at utsw.swmed.edu

Thanking you,
Yours
M.M.Rajan





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