CV Enclosed for Faculty and/or Research Positions.

Dr. JAI PARKASH jparkash at haverford.edu
Tue Sep 23 11:42:32 EST 1997


Covering Letter:
________________

From: Dr. JAI PARKASH,
Chemistry Department,
Haverford College,
Haverford, PA 19041 (USA)

Dear Sir/Madam'e:

I am enclosing a copy of my Curriculum Vitae including Names, Addresses,
Phone Numbers, Fax Numbers and E. Mail Addresses of my referees for your
kind consideration of the abovementioned position. At present, I am
working as a Postdoctoral Research Assistant in the Department of
Chemistry at Haverford College, Haverford, PA.  I am doing Postdoctoral
research work on the Resonance Light Scattering Spectroscopy of
Chlorophyll aggregates, Chlorosomes ( the light- harvesting system in
Photosynthetic Bacteria), Porphyrins aggregates and DNA / Porphyrins
aggregates as well as pseudoisocyanine dyes. The research work also
involves the Depolarized Resonance Light Scattering Spectroscopy of
these aggregated structures as a powerful tool to determine the overall
geometry of these aggregated structures. This work is being supported by
the National Science Foundation, USA.  I hope my extensive Postdoctoral
research works and trainings in the following fields would be  very
helpful :

(1) Molecular Biology (which also included heterologus gene expression
of anion exchanger AE2 protein in single transiently transfected cells
of known transfection status) and Biophysical Studies (that included the
determination of intracellular pH in these cells by using Ratio
Fluorescence Spectroscopy and Digital Video Imaging) during my stay at
the HARVARD UNIVERSITY, BOSTON (USA),

(2) Structural studies on the Photosystem II reaction center (including
isolation, purification and characterization and reconstitution of
Membrane Protein Complex) during my stay at the BROOKHAVEN NATIONAL
LABORATORY, UPTON, NY (USA),

(3) Protein Biochemistry research experiences (including Chemical
Modification of Functional Groups of Amino Acids in the Proteins) during
my stay at other institutions e.g., at the IMPERIAL COLLEGE OF SCIENCE,
TECHNOLOGY AND MEDICINE OF THE UNIVERSITY OF LONDON (U.K.), and

(4)  Resonance Light Scattering Spectroscopy and the Depolarized
Resonance Light Scattering Spectroscopy of molecular aggregates, during
my present stay at the HAVERFORD COLLEGE, HAVERFORD, PA (USA).

I will be eagerly looking forward to hear from you soon.

With best regards,

Yours sincerely,


(JAI PARKASH, Ph.D.)






 Dr. JAI PARKASH, Ph.D.,
 203 STOKES HALL,
 CHEMISTRY DEPARTMENT,
 HAVERFORD COLLEGE,
 HAVERFORD,  PA 19041

 PHONE: (610) 896-1325 (OFFICE) ; (610) 225-0185 (RESIDENCE)
 FAX: (610) 896-4904 (OFFICE)
 __________________________________________________________

___________________________________

Curriculum Vitae of Dr. Jai Parkash
___________________________________

JAI PARKASH, Ph.D.
290 AVON ROAD, APARTMENT # J - 408,
DEVON, PA 19333 - 1360

(610) 225-0185 (Res.); (610) 896-1325 (Office);
(610) 896-4904 (Fax, Office)
E.Mail; JPARKASH at HAVERFORD.EDU


Post-Doctoral Research Work Experiences:
________________________________________

Postdoctoral Research Assistant 1996-Present Chemistry Department
Haverford College
Haverford, PA 19041, (USA)

Senior Research Associate 1993 - 1996 Biology Department
Brookhaven National Laboratory
Upton, New York, N.Y. 11973, (USA)

Post-Doctoral Research Associate 1991-1993 Harvard University
Harvard Medical School
Boston, MA 02215, (USA)

Post-Doctoral Research Associate 1990-1991 Imperial College of Science,
Technology and Medicine
Department of Biochemistry
University of London
London, SW7 2AY, (England)

Post-Doctoral Research Associate 1988-1989 State University of New York
New York, N.Y. 13210, (USA)

Post-Doctoral Research Fellow 1987-1988 School of Life Sciences
Jawaharlal Nehru University
New Delhi, (India)


Educational Qualifications:
___________________________

Ph.D. (Life Sciences) 1980 - 1986 School of Life Sciences
Jawaharlal Nehru University
New Delhi, (India)

M.Phil. (Life Sciences) 1978 - 1980 School of Life Sciences
Jawaharlal Nehru University
New Delhi, (India)

M.Sc. (Life Sciences) 1976 - 1978 School of Life Sciences
Jawaharlal Nehru University
New Delhi, (India)

B.Sc. (Honours) Chemistry 1972 - 1975 Department of Chemistry
University of Delhi
Delhi, (India)


Topic of Ph.D. Thesis : Studies on Nucleotide Binding to Coupling Factor
1 and its Relation to Photophosphorylation.
_________________________________

Ph.D. Advisor : Professor G. S. Singhal
School of Life Sciences
Jawaharlal Nehru University
New Delhi, (India)


List of Publications:
_____________________

1. Jai Parkash and G. S. Singhal (1984) Effect of Chemical Modification
of Primary Amino Group of Salt Washed Thylakoid Membrane by
Fluorescamine on Different Parameters of Energy Transduction in
Chloroplast. Adv. Photosynth. Res. 2: 623-626
2. K. N. Singh, Jai Parkash, A. K. Agarwal and G. S. Singhal (1984)
Interaction of Herbicides With Pea Chloroplast. Z. Naturforsch 39c:
464-467
3. R. Paliwal, Jai Parkash and G. S. Singhal (1985) Trypsin Digestion of
Photosystem II Particles and the Effect of Calcium Ions on the Electron
Transport. Photochem. Photobiol. 41: 62s
4. Jai Parkash, Dinesh K. Sukumaran and G. S. Singhal (1986) Effect of
Divalent Cations on the Ageing of Wheat Chloroplast. Prog. in
Photosynth. Res. 2: 289-292
5. Jai Parkash and G. S. Singhal (1987) Localized vs Delocalized
Pathways of Proton Translocation. A study with Uncouplers and
Ionophores. Proc. Ind. Natl. Sci. Acad. B53: 435-438
6. G. S. Singhal and Jai Parkash (1987) Primary Photosynthetic Processes
and the Environmental Stress. International Symposium on Electron
Microscopy and Biophysics.
7. Jai Parkash and G. S. Singhal (1989) The Involvement of Suphydryl
Group of Coupling Factor 1 (CF1) in Energy Transduction in Chloroplast.
In: Photosynth. Molecular Biology and Bioenergetics (G. S. Singhal, J.
Barber, R. A. Dilley, Govindjee, R. Haselkorn and P. Mohanty eds)
239-246, Springer Verlag
8. R. Paliwal, Jai Parkash and G. S. Singhal (1989) Isolation of a
Highly Active PS II Preparation from Wheat (Triticum Aestivum) and the
Chemical Modification of Tyrosyl Residue with 4-Chloro-7-
Nitrobenzofurazan (NBD-Cl). In: Photosynth. Molecular Biology and
Bioenergetics (G. S. Singhal, J. Barber, R. A. Dilley, Govindjee, R.
Haselkorn and P. Mohanty eds) 163-174, Springer Verlag
9. Jai Parkash and G. S. Singhal (1989) Relationship Between Energy
Dependent Release of Adenine Nucleotide from CF1 and
Photophosphorylation. In: Techniques and New Developments in
Photosynthesis (J. Barber and R. Malkin eds) NATO-ASI Series 168,
275-280, Plenum Publishing Corp.
10. S. L. Alper, A. Stuart-Tilley, T. Lee and Jai Parkash (1992)
Fluorescence Analysis of Heterologus Gene Expression in Single
Transiently Transfected Cells of Known Transfection Status. Journal of
American Society of Nephrology, 2: 694a
11. L. Jiang, A. Stuart-Tilley, Jai Parkash and Seth L. Alper (1994) pHi
and Serum Regulate AE-2 Mediated Cl-/HCO3- in CHOP Cells of Defined
Transient Transfection Status. American Journal of Physiology, 267,
(Cell Physiology 36) C845-C856
12. Jai Parkash and A. M. Saxena (1995) Stabilization and Reconstitution
of Oxygen-Evolving Photosystem II Reaction Center. Biophysical Journal,
68, A92
13. Jai Parkash and J. C. DePaula (1997) Depolarized Resonance Light
Scattering Spectroscopy of Chlorophyll a Aggregates and Chlorosomes. In:
Fourteenth Annual USA Eastern Regional Photosynthesis Conference (Robert
Knox, Chair) 8, Abs # B2, Marine Biological Laboratories, Woods Hole,
Massachusetts, USA.
14. Jai Parkash, J. H. Robblee, J. Agnew, E. Gibbs, P. Collings, R. F.
Pasternack, and J. C. DePaula (1997) Depolarized Resonance Light
Scattering by Porphyrin and Chlorophyll a Aggregates. Manuscript under
Preparation (To be communicated to Biophysical Journal very soon).
15. J. C. DePaula, Jai Parkash and R. F. Pasternack (1997) Depolarized
Resonance Light Scattering Spectroscopy: Theory and Applications to
Porphyrin and Chlorophyll Aggregates. In: Gordon Research Conference,
USA, August 1997.

Research Skills:
________________

1. Spectroscopic Techniques- Time Resolved Fluorescence Spectroscopy
(picosecond and nanosecond time domains) including lifetimes of
chlorophyll a fluorescence; Difference Absorbance, Derivative
Spectroscopy, Single/Dual Wavelength Absorbance Spectroscopy;
Fluorescence, Fluorescence Induction, both at higher temperatures and
temperatures down to 77K. Fluorescence Polarization Spectroscopy,
Resonance Light Scattering Spectroscopy, Depolarized Resonance Light
Scattering Spectroscopy.
2. Digital Video Imaging of single living cells- use of Ratio
Fluorescence Spectroscopy Coupled to Microscopy. Image Acquisition,
Image Analysis, Image Storage including Image Maintenance Involving
Magneto-Optical High Speed Storage. Intracellular pH determination in
single living cells by fluorescence video imaging procedures.
3. Chromatography- Ion-Exchange, Gel Filtration, HPLC size exclusion
chromatography, Reverse Phase HPLC, GLC, TLC, Hydrophobic Interaction
Chromatography.
4. Electrophoresis- SDS-PAGE, Isoelectrofocussing.
5. Ultracentrifugation, Density Gradient Centrifugation.
6. Oxygen measurements, Photophosphorylation, Electron transport, PS II,
PS I and the whole chain,Charge Separation Activity, D pH and D U
Determinations.
7. Isolation, purification and characterization of CF1, PS II (including
D1/D2/Cytochrome b-559 complex).
8. Chemical modification of CF1, PS II (including PS II reaction
center).
9. Reconstitution of PS II in artificial liposomes.
10. Preparation of submitochondrial particles and chemical modification.

11. Cell Culture, Cell Transfection.
12. Dynamic Laser Light Scattering measurement.
13. Neutron diffraction.
14. Atomic absorption spectroscopy.
15. Scintillation Counting- solid as well as liquid scintillation
counting.


Summary of Research Work Done During Ph.D. :
____________________________________________

In order to understand the basic mechanism of photophosphorylation, I
had carried out a detailed analysis of the relationship between energy
dependent release of adenine nucleotides and photophosphorylation. The
techniques of Protein Chemistry and Photosynthesis were utilized to
probe into the active site of coupling factor 1 (CF1) ATPase involved in
these processes. In this context, the chemical modification of the
following functional groups of CF1 in situ were carried out and their
effects on the energy dependent release of adenine nucleotides and
photophosphorylation were studied:

(1) e- amino group of lysine residues by Fluorescamine,
(2) phenolic -OH group of tyrosine residues by NBD-Cl,
(3) -COOH groups of acidic amino acids residues by DCCD and
(4) -SH group by NEM and Iodosobenzoate as well as the reversal effect
by DTT.

The effects of several uncouplers, ionophores and energy transfer
inhibitors which affect both the energy dependent release and
photophosphorylation were also studied. The main results and conclusions
arrived at are briefly described below:

(a) Active site for the synthesis of ATP and the binding site for the
adenine nucleotide are present at different location on CF1 though these
sites interact with each other.
(b) e- amino group of lysine residues which get exposed during the high
energy state, -COOH group of acidic amino acid residues and phenolic -OH
group of tyrosine residues are involved at the active site of CF1 for
ATP synthesis.
(c) Thiol modulation of -SH group of CF1 regulates both the activation
and the catalytic functions of CF1.
(d) The electrochemical gradient of protons is the driving force for
both the activation of CF1 and ATP synthesis by CF1.


Other Research Activities:
__________________________

In addition to my doctoral research work as mentioned above, I have also
carried out research work in several other research projects which were
supported by USDA, NIH, DOE (USA) and Agriculture and Food Research
Council of United Kingdom. The nature of the research work carried out
in these different projects is briefly described below:

1. Determination of intracellular pH and chloride-bicarbonate exchange
in normal culture cells (COS cells, CHOP cells, MDCK cells, HeLa cells)
as well as in transietly transfected (with cDNA for anion exchanger
protein AE2). This work involved the setting up the Digital Video
Imaging and Ratio Fluorescence Spectrophotometry.
2. The electron donor side of PS II in the D1/D2/Cytochrome b-559
complex with particular reference to the involvement of Manganese
binding and chemical modification of histidine residues by
diethylpyrocarbonate in order to locate the Manganese binding sites.
3. Chemical modification studies on the F0 part of coupling factor
complex from beef heart mitochondria in order to analyse the
relationship between proton translocation across the energy transducing
membrane of mitochondria and ATP synthesis/hydrolysis. Role of -COOH
group of F0 in the long range interaction between F0 and F1.
4. The process of ageing of isolated thylakoid membranes and the
influence of divalent cations, cholesterol, temperature and light and
dark conditions on the ageing process. Use of DPH as extrinsic probe and
Chlorophyll a as intrinsic fluorescence probe to analyse the changes in
the fluidity of the thylakoid membranes during ageing.
5. Effects of herbicides e.g., Metribuzin, Pyrazon and Triazole on the
partial reactions of photosynthesis in pea chloroplasts.
6. Isolation, purification and characterization of PS II reaction
center, LHCPC a/b protein from higher plants and the effects of
temperature, trypsin digestion and divalent cations on the spectroscopic
properties and functional activities.
7. Chemical modification studies on the involvment of tyrosine residues
on the electron donor side of highly active PS II particles isolated
from spinach and wheat.
8. Isolation, purification and characterization of PS II reaction center
(D1/D2/Cytochrome b-559 complex and D1/D2 complex) from a thermophillic
cyanobacterium Phormidium laminosum. The research work also involved the
reconstitution of PS II reaction center into artificial lipid bilayer
and the structure determination of PS II reaction center by using the
methodologies of Neutron Diffraction and Membrane Protein
Reconstitution.


Present Research Activities:
____________________________

At present, I am doing research work on the Resonance Light Scattering
Spectroscopy of Chlorophyll a aggregates, Chlorosomes ( the light-
harvesting system in Photosynthetic Bacteria), Porphyrins aggregates and
DNA / Porphyrins aggregates as well as pseudoisocyanine dyes. The
research work also involves the Depolarized Resonance Light Scattering
Spectroscopy of these aggregated structures as a powerful tool to
determine the overall geometry of these aggregated structures. This work
is being supported by the National Science Foundation, USA.


Other Academic Affiliations:
____________________________

At the moment, I am also a Visiting Senior Research Associate at the
Biology Department of the Brookhaven National Laboratory, Upton, New
York, USA of the United States Department of Energy.


Memberships:
____________

1. American Society of Plant Physiology.

2. American Chemical Society.

3. American Association for the Advancement of Science.

4. International Society of Photosynthesis Research.


References:
___________

Names, Addresses, Phone Numbers, Fax Numbers and E. Mail Addresses of my
referees are as follows:

1.  Dr.  J. C. dePAULA,
Associate Professor and Chairman,
Department of Chemistry,
HAVERFORD COLLEGE,
370 Lancaster Avenue,
Haverford, PA 19041-1392    (USA)

PHONE: (610) 896-1217

FAX: (610) 896-4904

E.Mail: jdepaula at haverford.edu



2.  Dr.  Seth L. ALPER, M.D., Ph.D.,
Associate Professor of Medicine (Cell Biology),
HARVARD MEDICAL SCHOOL,  HARVARD UNIVERSITY,
BETH ISRAEL DEACONESS MEDICAL CENTER,
Molecular Medicine and Renal Units,
Room RW-763 East Campus,
330 Brookline Avenue,
Boston, MA 02215  (USA)

PHONE: (617) 667-2930

FAX: (617) 667-2913

E.MAIL:  SALPER at MERCURY.BIH.HARVARD.EDU
E.MAIL:  SALPER at BIDMC.HARVARD.EDU



3.  Dr.  D. K. SUKUMARAN,
Associate Professor,
Department of Chemistry,
309 Natural Sciences Complex,
STATE UNIVERSITY OF NEW YORK  at Buffalo,
Buffalo, N.Y. 14260   (USA)

Phone: (716) 645-6800 ext 2088

FAX: (716) 645-6963

E.Mail: dks at acsu.buffalo.edu
E.Mail: dinesh at silicon.chem.buffalo.edu



4.  Dr.  D.  ROY,
Associate Professor,
Department of Environmental Health Sciences,
School of Public Health,
THE UNIVERSITY OF ALABAMA AT BIRMINGHAM,
Fourth Floor Tidwell Hall, 720 South 20th Street,
Birmingham, Alabama  35294-0008   (USA)

PHONE: (205) 934-6081

FAX: (205) 975-6341

E.MAIL: ROYD at CRL.SOPH.UAB.EDU
E.MAIL: ROYD at TIDWELL.SOPH.UAB.EDU


5.   Dr.  ROBERT  M. PASTERNACK,
The Edmund Allen Professor of Chemistry,
Department of Chemistry,
SWARTHMORE COLLEGE,
Swarthmore, PA 19081   (USA)

Phone:  (610) 328-8559

Fax: (610) 328-7355

E.MAIL:  RPASTER1 at SWARTHMORE.EDU



6.  Dr.  A. M. SAXENA,
Biophysicist,
BROOKHAVEN NATIONAL LABORATORY,
Upton, N.Y. 11973    (USA)

Phone: (516) 344-4844

FAX: (516) 344-3407

E.MAIL:  SAXENA at BNLBIO.BIO.BNL.GOV

____________________________________________________________




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