Green Fluorescent Protein Purificaton Short Course

Daniel Gonzalez meton at rci.rutgers.edu
Fri Apr 3 13:28:18 EST 1998


I have been asked to announce the following,

                  The State University of New Jersey
                                Rutgers
                        Campus at New Brunswick

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material

                            June 7-12, 1998,
                            July 12-17, 1998,and
                            August 2-7, 1997

More than 500 scientists from around the world have strongly recommended this
intensive course as an opportunity to develop protein research and analytical
skills in a retreat setting.  Participants work hard, identify and solve
problems in the lab and enjoy camaraderie and good food and beer with
colleagues.

This five-day laboratory course covers a wide variety of conventional methods
for protein isolation, purification, and characterization. The course format
integrates hands-on laboratory exercises with classroom lectures,
demonstrations, study breaks, and short take-home assignments.

A special feature of the course is that all laboratory work will be performed
on the same starting sample (Aequorea GFP*), which will be purified from an
exceedingly crude form to near homogeneity as judged by high performance
liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing,
and capillary zone electrophoresis. This feature provides a continuity of
purpose, integrating dozens of preparative and analytical protein techniques
in a way that few competing courses can match.

A problem-solving approach will be used throughout the course. Under the
guidance of experienced lab instructors, participants will work in groups
of three to plan their own protocols, analyze data, and interpret results.
A student-teacher ratio not greater than 8:1 will be maintained and the
faculty coordinators will be present throughout the course.

*Note: The use of GFP from a recombinant source (E. coli) is also being used
 as starting material due to its popularity within the scientific community.

For further details you can reach us,

by E-mail at:  meton at rci.rutgers.edu
by phone at:  (908) 932-9071 extension 219
by FAX at:  (908) 932-8965

for a brochure and further information please visit the GFP purification
short course official Web site at:
http://www.rci.rutgers.edu/~meton/protein.html




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