DEPC

bli1997 at yahoo.com bli1997 at yahoo.com
Fri Apr 24 00:05:05 EST 1998



> >
>
> DEPC is broken down to CO2 and H2O after autoclaving
>
> Time for binding RNases: it is common practice to add 0.1% v/v DEPC to water,
> let it stir overnight, then autoclave it in the morning.  After autoclaving
> the water may still have a "bubble gum" scent. This is now DEPC treated water
> with no active RNAses but RNases can still be reintroduced since the DEPC is
> no longer present/active
>
> DEPC is VERY TOXIC and should be added to any solutions in a proper
> fumehood!!!
>
 Hi, can you further explain how toxic DEPC is and how it does harm to us?
Thank you.

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