plasmid question

Shi Dingji cyanoshi at PUBLIC.BTA.NET.CN
Thu Aug 20 08:38:00 EST 1998

I have a  plasmid about 2.8kd which is easily handled several months
ago. But now it has gone wrong. The problems are as follows:

1. It replicates poorly now.
2.The result of single enzyme digestion is not complete any more.
3. When I use two enzymes to digest it, I can't see the small fragment I
want any longer. Moreover the relatively large fragment is not only one.

What's the matter? I have been trapted in it many days. I really got
headache on it.

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