pcboom.andrea at flashnet.it
Sat Aug 22 14:59:32 EST 1998
Just guessing, my first thought was an insertion problem. Maybe the
mutation happened in/near the replication region and affected also one
restriction site, thus causing the "elongation" of the previous short
You should give some more info:
what kind of plasmid is it ?
where it is mantained ?
what sites does it contains (e.g. polilinker, ori-c, boxes, etc) ?
SeeYa, Andrea Galli
> I have a plasmid about 2.8kd which is easily handled several months
> ago. But now it has gone wrong. The problems are as follows:
> 1. It replicates poorly now.
> 2.The result of single enzyme digestion is not complete any more.
> 3. When I use two enzymes to digest it, I can't see the small fragment I
> want any longer. Moreover the relatively large fragment is not only one.
> What's the matter? I have been trapted in it many days. I really got
> headache on it.
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