FISH problem

flmv1v at flmv1v at
Mon Feb 2 05:54:26 EST 1998


I have started doing FISH experiment. I have a 
problem with the final morphology of the 
chromosomes. They appear phffy and enlarged. They 
lose any distinctive features sometimes fusing in 
one mass. I believe that the denaturation step is 
the problem. I have reduced the temperature but 
without any significant changes. Is there any 
thing that I should do to avoid that? Any 
technical help of referal to the appropriate 
newsgroup is welcome.


F. Vaillant

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