gold conjugate

Thierry Leclipteux thierry at stat.fmv.ulg.ac.be
Fri Feb 13 08:04:47 EST 1998


I have a problem in conjugating a monoclonal antibody to 20 nm gold
particles.  Monoclonal pi is close to 6.5 and several trials have been
performed at ph 7, 8 and even 9 without significant increase in the
conjugate sensitivity.  In our procedure 0.01 mg IgG are incubated with
1 ml of gold particules (at 0.01 %).
I am looking for a protocol which enables me to increase this conjugate
sensitivity.
Thanks for replying either in the news or by e-mail.



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