Very Quick Cell Question
UncleAl0 at ix.netcom.com
Sun Feb 15 10:42:32 EST 1998
TODD J PIERCE wrote:
> I know I can see individual strands of bacteria at 100x with a microscope.
> My question is this what magnification do I need to see both a cell and
> its nucleus--that is, for a fairly large cell--200x, 500x? In our very
> basic lab, we have microscopes up to 100x. Are microscopes with higher
> ranges of magnifacation common in working labs?
> Could I see the nucleus with the standard microscope, or can one only see
> the dark outline of the nucleus with an electron microscope?
If you want to locate the nucleus you want it to call out to you above
background. Use a fluorescent stain for double strand nucleic acid.
Will ethidium bromide (toxic!) do it (under near-UV illumination)? Ask
the experts: http://www.probes.com/
Visualizing cell nuclei and chromosomes arrested by Colcemide(tm) or
colchicine is standard operating procedure. It doesn't require anything
beyond an optical microscope. Observing onion root tip chromosomes this
way is a high school lab prep.
Uncle Al Schwartz
UncleAl0 at ix.netcom.com ("zero" before @)
(Toxic URLs! Unsafe for children, Democrats, and most mammals)
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