cDNA vs Genomic clones for Mammalian Expression

Mr JG Evans jgevans at hgmp.mrc.ac.uk
Wed Jan 14 07:13:20 EST 1998


Can anyone point me in the direction of a reference stating whether comic clones produce higher expression levels in transfected mammalian cells?
I am currently obtaining quite low expression (5-10ng/ml) using a genomic
clone in pcDNA3 transiently transfected into CHO cells (using perfect lipids).
Thanks for your help!
James
j.evans at ed-rbu.mrc.ac.uk




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