necessity of sequencing

Jay Mone' jmone at MARAUDER.MILLERSV.EDU
Mon Nov 16 11:27:26 EST 1998


I agree with Michael Lush.  Unless you used a high fidelity polymerase 
for PCR (Pfu or a similar DNA pol), it is very likely that the 
sequence may contain errors.  If this is an expression vector, it 
could really screw things up.

Jay Mone'



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